University of Wisconsin-Madison/Promega Corporation
Symposium on Biological Imaging

Solving Biological Problems with Imaging Technology

 


Katherine Kalil and Erik Dent.jpg (2037860 bytes)

Hamster cortical neuron and a glial cell in culture.  Triple labelled with phalloidin (red) to label filamentous actin, an antibody to tyrosinated tubulin (green) to stain newly polymerized microtubules and an antibody to acetylated tubulin (blue) to stain older microtubles.

Click on image to view full size.

 


Sponsors

W. M. Keck Laboratory for Biological Imaging

UW-Madison, Laboratory of Molecular Biology

Promega Corporation

DATE AND
LOCATION

Friday, May 25, 2001

The Biopharmaceutical
Technology Center at
Promega Corporation
5445 East Cheryl Pkwy.
Madison,  WI  53711

Introduction


Keynote Address
Scott Fraser, Ph.D.

GENERAL
INFORMATION

new.gif (116 bytes)Photos of the event.

Program
List of Speakers

Workshop Descriptions


Abstract Form
Deadlines

Hotel Information
Driving Directions

keck..jpg (77688 bytes)

Rat lung, cold-preserved for organ transplantation, stained to distinguish living (green; calcium AM; indicator of intracellular esterase activity) cells from dead (red; ethidium homodimer-1; indicator of compromised plasma membrane) cells.

Image appeared on Cover of BioTechniques 30(2)


  Click on images to view full size.

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Primary cells were isolated from the embryonic rat striatum and allowed to differentiate into neurons. Cells were fixed and immunostained with a monoclonal anti-b III tubulin (Cat# G7121), followed by and incubation with a secondary Cy-3TM conjugated anti-mouse IgG.

Human neuroblastoma SH-SY5Y cells treated with staurosporine are labeled with Anti-PARP p85 Fragment pAb (red) and Anti-bIII tubulin mAb (green). Only the apoptotic cells are labeled with the Anti-PARP p85 Fragment pAb. 

Featured on the Cover of April, 2001 BioTechniques, Vol 30, No. 4.

3-photon image of DAPI-stained cell nuclei in C.elegans larva.

3D volume rendering of an embryonic Xenopus spinal cord and notochord (below) double-labeled for tubulin (red) and actin (green).


Organizers:  Ronald E. Kalil, Ophthalmology and Visual Sciences, University of Wisconsin-Madison;
John G. White, Laboratory of Molecular Biology, Department of Anatomy, University of Wisconsin-Madison;
Robert F. Bulleit, Promega Corporation

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